2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

doi:10.11843/j.issn.0366-6964.2018.08.025

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1789-1796.doi: 10.11843/j.issn.0366-6964.2018.08.025

2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

蒋大秀¹, 马静¹, 王晓泉^1,2, 胡顺林^1,2, 刘晓文^1,2*, 刘秀梵^1,2

1. 扬州大学兽医学院农业部畜禽传染病重点开放实验室, 扬州 225009;
2. 江苏省重点动物传染病和人畜共患病预防控制联合创新中心, 扬州 225009

收稿日期:2018-03-05 出版日期:2018-08-23 发布日期:2018-08-23
通讯作者: 刘晓文,硕士生导师,副教授,E-mail:phontom226@qq.com
作者简介:蒋大秀(1992-),男,山东菏泽人,硕士,主要从事H9N2亚型禽流感流行病学调查及致病机制研究,E-mail:349456530@qq.com
基金资助:
“十二五”农村领域国家科技计划（2015BAD12B01-3）

Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017

JIANG Da-xiu¹, MA Jing¹, WANG Xiao-quan^1,2, HU Shun-lin^1,2, LIU Xiao-wen^1,2*, LIU Xiu-fan^1,2

1. Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Key Joint Center of Infectious Diseases and Zoonosis Prevention and Control of Jiangsu Province, Yangzhou 225009, China

Received:2018-03-05 Online:2018-08-23 Published:2018-08-23

摘要/Abstract

摘要：

活禽市场的家禽，特别是水禽在禽流感病毒（avian influenza virus，AIV）的演化、维持和扩散过程中起重要作用，因此对活禽市场进行长期不间断的流行病学监测非常重要。2016年10月至2017年9月，每月定期采集华东地区某活禽市场家禽喉头和泄殖腔棉拭子，并接种10日龄SPF鸡胚，测定鸡胚尿囊液HA效价，取阳性尿囊液进行HI试验分型，应用RT-PCR对该样品进行全基因PCR并测序，利用DNASTAR、MEGA6.0分析序列，并绘制系统发生树。结果共采集样品1 178份，来自127群，H9N2 AIV阳性样品100份。从水禽中分离到H9N2 AIV 7株，对7株病毒全基因组进行测序和遗传进化分析，不同基因片段均存在不同的遗传进化趋势。活禽市场水禽体内携带的7株H9N2 AIV与当前家禽优势流行基因型一致，因此对活禽市场家禽中循环存在的AIV进行的定期监测，对了解潜在的流行株的演化和出现有重要作用。

Abstract:

Domestic poultry in live poultry markets (LPMs), especially waterfowl, play an important role in the evolution, maintenance and spread of the avian influenza viruses (AIVs). Therefore, it is important to conduct a long-term, regular epidemiological surveillance in the LPMs. From October 2016 to September 2017, we collected oropharyngeal and cloacal swabs from the poultry traded in the LPMs in the eastern China monthly. The samples were inoculated into SPF embryonated chicken eggs. Viruses in the allantoic fluid were confirmed by HA test and were divided to different subtypes by HI test with subtype-specific antibodies. Then eight segments of AIVs' genome were amplified by RT-PCR and these segments were sequenced. Sequence alignment and phylogenetic analysis were performed by software such as DNASTAR and MEGA6.0. A total of 1 178 samples from 127 flocks were collected, and 100 H9N2 AIV strains were isolated, among which 7 strains came from waterfowl. Genetic analysis of eight segments showed that all 7 strains belonged to the S genotype H9N2 AIVs. The genotype of H9N2 viruses from waterfowl traded in LPMs was consistent with that of predominant strains which were circulating in chickens in China. Our study highlights the need to constantly monitor the H9N2 viruses in poultry in LPMs to understand the emergence and evolution of strains that may become epidemic.

中图分类号:

S852.659.5

蒋大秀, 马静, 王晓泉, 胡顺林, 刘晓文, 刘秀梵. 2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析[J]. 畜牧兽医学报, 2018, 49(8): 1789-1796.

JIANG Da-xiu, MA Jing, WANG Xiao-quan, HU Shun-lin, LIU Xiao-wen, LIU Xiu-fan. Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1789-1796.

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2016-2017年华东地区活禽市场7株H9N2禽流感病毒遗传进化分析

Genetic Evolution of Seven H9N2 Avian Influenza Viruses Isolated from Live Poultry Market in Eastern China During 2016-2017

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